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우수성과SAIHST 구성원의 언론보도내용 및 수상내역, 각 연구분야의 우수 학술지에 게재된 논문 등 우수한 성과들을 소개합니다.

[조동규 교수/ 우수논문] Journal of Extracellular Vesicles. 9 Mar 2020
No -1
작성자 관리자
등록일 2020/04/27


책임저자

 


조동규 교수

SAIHST 융합의과학과/ 약학대학 약학과

 

 

Impact Factor('18)= 11.000 

 

 


Journal of Extracellular Vesicles. 2020 Mar 9;9(1):1735249.  doi: 10.1080/20013078.2020.1735249. 

 

Small Extracellular Vesicles From Human Adipose-Derived Stem Cells Attenuate Cartilage Degeneration

Chang Hee Woo, Hark Kyun Kim, Gun Young Jung, Youn Jae Jung, Kyoung Soo Lee, Ye Eun Yun, Jihoon Han, Jeongmi Lee, Woo Sung Kim, Ji Suk Choi, Siyoung Yang, Jae Hyung Park, Dong-Gyu Jo, Yong Woo Cho

 

Abstract

Osteoarthritis (OA) is a chronic degenerative disease of articular cartilage that is the most common joint disease worldwide. Mesenchymal stem cells (MSCs) have been the most extensively explored for the treatment of OA. Recently, it has been demonstrated that MSC-derived extracellular vesicles (EVs) may contribute to the potential mechanisms of MSC-based therapies. In this study, we investigated the therapeutic potential of human adipose-derived stem cells EVs (hASC-EVs) in alleviating OA, along with the mechanism. EVs were isolated from the culture supernatants of hASCs by a multi-filtration system based on the tangential flow filtration (TFF) system. The isolated EVs were characterised using dynamic light scattering (DLS), transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and flow cytometry analysis. The hASC-EVs not only promoted the proliferation and migration of human OA chondrocytes, but also maintained the chondrocyte matrix by increasing type Ⅱ collagen synthesis and decreasing MMP-1, MMP-3, MMP-13 and ADAMTS-5 expression in the presence of IL-1β in vitro. Intra-articular injection of hASC-EVs significantly attenuated OA progression and protected cartilage from degeneration in both the monosodium iodoacetate (MIA) rat and the surgical destabilisation of the medial meniscus (DMM) mouse models. In addition, administration of hASC-EVs inhibited the infiltration of M1 macrophages into the synovium. Overall results suggest that the hASC-EVs should be considered as a potential therapeutic approach in the treatment of OA.

         

Keywords: Extracellular vesicles; human adipose-derived stem cells; immune regulation; osteoarthritis.  

 

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